CD53 antibody (FITC)

Catalog No. ABIN1821089

The Mouse Monoclonal anti-CD53 antibody has been validated for FACS. It is suitable to detect CD53 in samples from Human.

Quick Overview for CD53 antibody (FITC) (ABIN1821089)

Target: CD53

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD53 antibody is conjugated to FITC

Application: Flow Cytometry (FACS)

Clone: 63-5A3

Product Details anti-CD53 Antibody (FITC)

Specificity: Human CD53.

Purification: Affinity purified

Immunogen: Human Sezary cells.

Isotype: IgG2b

Application Details

Application Notes: Approved: Flo

Usage: Flow Cytometry: 5x10e5 cultured human Jurkat cells per tube were incubated 45 min on ice with 80 μL of anti-CD53 antibody at 10 μg/mL. Cells were washed twice and incubated with Goat anti-Mouse IgG/FITC, after which they were washed three times, fixed and analyzed by FACS. Cells stained positive with a mean shift of 1.37 log10 fluorescent units when compared to a Mouse IgG2b negative control at a similar concentration. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 50 mM sodium phosphate,  pH 7.5, 100 mM potassium chloride, 150 mM sodium chloride

Handling Advice: Aliquot to avoid repeated freezing and thawing. Protect from light.

Storage: 4 °C,-20 °C

Storage Comment: Short term: store at 4°C. Long term: aliquot and store -20°C. Avoid freeze-thaw cycles. Protect from light.

Target Details for CD53

Target: CD53

Alternative Name: CD53

Background: Name/Gene ID: CD53
Family: Tetraspan

Synonyms: CD53, CD53 antigen, CD53 Molecule, CD53 tetraspan antigen, Cell surface antigen, Leukocyte surface antigen CD53, Transmembrane glycoprotein, Tetraspanin-25, Tspan-25, CD53 glycoprotein, Cell surface glycoprotein CD53, MOX44, TSPAN25

Gene ID: 963

UniProt: P19397